How Free Cationic Polymer Chains Promote Gene Transfection download
How Free Cationic Polymer Chains Promote Gene Transfection download
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Author: Yue Yanan
Page Count: 112 pages
Published Date: 03 Jun 2013
Publisher: Springer International Publishing AG
Publication Country: Cham, Switzerland
Language: English
ISBN: 9783319003351
File size: 24 Mb
Download Link: How Free Cationic Polymer Chains Promote Gene Transfection
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In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted "proton sponge" model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.
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Shakugan No Shana: v. 6 downloadPDF, EPUB, MOBI
How Free Cationic Polymer Chains Promote Gene Transfection. Yue Yanan
Author: Yue Yanan
Page Count: 112 pages
Published Date: 03 Jun 2013
Publisher: Springer International Publishing AG
Publication Country: Cham, Switzerland
Language: English
ISBN: 9783319003351
File size: 24 Mb
Download Link: How Free Cationic Polymer Chains Promote Gene Transfection
---------------------------------------------------------------
In this PhD thesis, Yue Yanan addresses a long-overlooked and critical question in the development of non-viral vectors for gene delivery. The author determines that those uncomplexed and cationic polymer chains free in the solution mixture of polymer and DNA facilitate and promote gene transfection. Furthermore, by using a combination of synthetic chemistry, polymer physics and molecular biology, Yue confirms that it is those cationic polymer chains free in the solution mixture, rather than those bound to DNA chains, that play a decisive role in intracellular trafficking. Instead of the previously proposed and widely accepted "proton sponge" model, the author's group propose a new hypothesis based on the results of several well-designed and decisive experiments. These results show that free polycationic chains with a length of more than ~10 nm are able to partially block the fusion between different endocytic vesicles, including the endocytic-vesicle-to-endolysosome pathway. This thesis is highly original and its results greatly deepen our understanding of polymer-mediated gene transfection. More importantly, it provides new insights into the rational design of next-generation superior polymeric gene-delivery vectors.
Read online How Free Cationic Polymer Chains Promote Gene Transfection Buy and read online How Free Cationic Polymer Chains Promote Gene Transfection Download How Free Cationic Polymer Chains Promote Gene Transfection ebook, pdf, djvu, epub, mobi, fb2, zip, rar, torrent Download to iPad/iPhone/iOS, B&N nook How Free Cationic Polymer Chains Promote Gene Transfection
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